Inactive

REQUEST FOR INFORMATION: 36C262-21-Q-0936. THIS REQUEST FOR INFORMATION (RFI) IS ISSUED SOLELY FOR INFORMATION AND PLANNING PURPOSES ONLY AND DOES NOT CONSTITUTE A SOLICITATION. THE SUBMISSION OF PRIC... REQUEST FOR INFORMATION: 36C262-21-Q-0936. THIS REQUEST FOR INFORMATION (RFI) IS ISSUED SOLELY FOR INFORMATION AND PLANNING PURPOSES ONLY AND DOES NOT CONSTITUTE A SOLICITATION. THE SUBMISSION OF PRICING, CAPABILITIES FOR PLANNING PURPOSES, AND OTHER MARKET INFORMATION IS HIGHLY ENCOURAGED AND ALLOWED UNDER THIS RFI IN ACCORDANCE WITH (IAW) FAR 15.201(e). This is not a solicitation announcement.  This is sources sought synopsis only for market research purposes ONLY. The purpose of this synopsis is to gain knowledge of potential qualified Service-Disabled Veteran Owned Small Businesses (SDVOSB) or Veteran Owned Small Businesses (VOSB) for relative to NAICS 339112 sources (size standard of 1000 employees). Responses to this synopsis will be used by the Government to make appropriate acquisition decisions. After review of the responses to this source sought synopsis, a solicitation announcement may be published in the FedBizOpps or GSA eBuy websites. Responses to this sources sought synopsis are not considered adequate responses to the solicitation announcement. All interested offerors will have to respond to the solicitation announcement in addition to responding to this source sought announcement.  The Department of Veterans Affairs, Network 22 Contracts Office, is seeking sources to provide: CHROMIUM CONTROLLER EQUIPMENT for the VA San Diego Healthcare System. If you are interested and can provide the required supplies, please provide the requested information as indicated below. Responses to this notice should include your: DUNS Number, Size of business, Company name, Address, Point of contact, Pursuant to the following questions: (1) Please indicate the size status and representations of your business, such as but not limited to: Service-Disabled Veteran Owned Small Business (SDVOSB), Veteran Owned Small Business (VOSB), Hubzone, Woman Owned Small Business (WOSB), Large Business, etc.)? (2) Is your company considered small under the NAICS code identified under this RFI? (3) Are you the manufacturer or distributor of the items being referenced above (or equivalent product/solution)? What is the manufacturing country of origin of these items? (4) If you re a small business and you are an authorized distributor/reseller for the items identified above (or equivalent product/solution), do you alter; assemble; modify; the items requested in any way? If you do, state how and what is altered; assembled; modified? (5) Does your company have an FSS contract with GSA or the NAC or are you a contract holder with NASA SEWP or any other federal contract? If so, please provide the contract type and number. (6) If you are an FSS GSA/NAC or NASA SEWP contract holder or other federal contract holder, are the referenced items/solutions available on your schedule/contract? (7) Please provide general pricing for your products/solutions for market research purposes. (8) Please submit your capabilities regarding the salient characteristics being provided and any information pertaining to brand name or equal to items to establish capabilities for planning purposes? Responses to this notice shall be submitted via email to: John.harrison2@va.gov Telephone responses shall not be accepted. Responses must be received no later than Monday, 07/07/2021 at 11:00 AM PST. If a solicitation is issued it shall be announced later, and all interested parties must respond to that solicitation announcement separately from the responses to this request for information. Responses to this notice are not a request to be added to a prospective bidders list or to receive a copy of the solicitation. Please provide unofficial pricing. Notice: No remanufactures or gray market items/ supplies will not be acceptable. Interested parties of RFI must be an authorized reseller, distributor, or dealer of the O.E.M. (Original Equipment Manufacturer). Verification can be provided by an authorization letter or other documents from the OEM. Equipment shall be in accordance with the OEM terms and conditions. Salient Characteristics The system should have the following salient characteristics: Single Cell Partitioning for Transcriptome Analysis: The Chromium Next GEM Single Cell Gene Expression assay enables single cell transcriptional profiling of up to millions of single cells. Single cell suspensions loaded onto the instrument are partitioned into GEMs, where transcripts are tagged with cell specific barcodes. The barcoded cDNA is then pooled for downstream processing and library preparation. In addition, the assay enables targeting using predesigned or customized gene panels, as well as simultaneous assessment of gene expression and feature profiling (e.g. cell surface proteins, guide RNA), enabling enhanced phenotypic profiling in thousands of individual cells. The Chromium Single Cell Gene Expression assay contains reagents to construct a library using the Feature Barcoding technology. However, compatible input materials, such as, cell surface proteins labeled with antibody-oligonucleotide conjugates, CRISPR transduced cells or any other products need to be prepared by the users. Our precise and efficient microfluidics allow 100- 80,000+ cells to be recovered in droplets in each <18-minute run with a low doublet rate, facilitating the profiling of precious and rare cell populations. After sequencing, downstream bioinformatics tools use the cellular barcodes to group transcripts that originated from the same cell, revealing the transcriptome and/or the feature information of each individual cell. Single Cell Partitioning for Immune Profiling Analysis: The Chromium Next GEM Single Cell Immune Profiling Solution is a comprehensive approach to simultaneously examine the cellular context of the adaptive immune response and immune repertoires of hundreds to tens of thousands of T and B cells in human or mouse on a cell-by-cell basis. Single cell suspensions loaded onto the instrument are partitioned into GEMs, where transcripts are tagged with cell specific barcodes. The barcoded cDNA is then pooled for downstream processing and library preparation. The assay enables simultaneous examination of the cellular context of the adaptive immune response and immune repertoires of hundreds to tens of thousands of T and B cells in human or mouse. These libraries can be enriched for specific targets using pre-designed or customized gene panels. Further, they can be combined with the Feature Barcoding technology, detection and analysis of additional cellular features, such as, cell surface proteins and antigen specificity to enhance immune cell phenotyping and study dynamic interactions between lymphocytes and target cells is enabled. Precise and efficient microfluidics allow 500-80,000+ cells to be recovered in droplets in each <18-minute run with a low doublet rate, facilitating the profiling of precious and rare cell populations. After sequencing, downstream bioinformatics tools use the cellular barcodes to group transcripts that originated from the same cell, revealing the transcriptome and/or the full-length T- or B-cell receptor sequences and additional cell feature information of each individual cell. Single Nuclei Partitioning for Epigenome Analysis: The Chromium Controller enables the analysis of chromatin accessibility at single cell resolution. In the Chromium Next GEM Single Cell Assay for Transposase Accessible Chromatin (ATAC) workflow, single nuclei are first treated in bulk with transposase enzyme to preferentially insert sequencing adaptors into accessible DNA regions, and then are partitioned into GEMs in the Chromium Controller. All accessible DNA fragments from the same nucleus share a common 10x barcode. The barcoded, accessible DNA fragments are subsequently pooled for downstream processing and library preparation. The precise and efficient microfluidics allow 500-80,000+ nuclei to be recovered in droplets in each <18-minute run with a low doublet rate, facilitating the open chromatin profiling of challenging and rare cell populations. The barcoded library fragments can then be easily traced back to the open chromatin landscape for each nucleus from which they originated using intuitive bioinformatics tools. Single Nuclei Partitioning for Combined Transcriptome and Epigenome Analysis: The Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Solution enables simultaneous profiling of the transcriptome and epigenome in the same nucleus across hundreds to tens of thousands of nuclei. Single nuclei suspensions are first treated in bulk with transposase enzyme to preferentially insert sequencing adaptors into accessible DNA regions, and then are partitioned into GEMs in the Chromium Controller. Transcripts and accessible DNA regions are labeled with cell-specific barcodes in the GEMs, followed by pooling and downstream library construction to generate two paired NGS libraries one gene expression library from single cells, and one Assay for Transposase Accessible Chromatin (ATAC) library from the same single cells. The precise and efficient microfluidics allow 500-80,000+ nuclei to be recovered in droplets in each <18-minute run with a low microfluidic multiplet rate, facilitating simultaneous analysis of gene expression and accessible chromatin from challenging and rare cell populations. After sequencing, downstream bioinformatics tools use the cellular barcodes to group transcripts and accessible DNA fragments that originated from the same cell, revealing the transcriptome and paired epigenome of each individual cell. Next GEM Single Cell Gene Expression Solution Summary: The Chromium Next GEM Single Cell Gene Expression Solution delivers a scalable microfluidic platform for 3 digital gene expression profiling of up to 80,000 cells per run. In addition, the Feature Barcoding technology enables simultaneous profiling of additional cellular phenotypes (e.g. cell surface proteins, gRNAs). The 10x Next GEM Technology samples a pool of ~3.6 million barcodes to separately index each cell s transcriptome. It does so by partitioning thousands of cells into nanoliter-scale GEMs, in which all generated cDNAs share a common barcode. Libraries are generated and sequenced from the cDNA, and barcodes are then used to associate individual reads back to their individual partitions or cells. The Chromium Controller provides a complete solution for uncovering cell-to-cell gene expression and identifying rare cells in heterogeneous populations. Technical innovations and the Feature Barcoding technology allows the efficient creation of high complexity libraries from single cells to maximize information from any sample. Workflow: The Chromium Controller uses a microfluidic chip to provide high-throughput reagent delivery. The 8- sample cartridge is loaded with Gel Beads, Single Cell 3 reagents, a cell suspension and an oil surfactant solution. Reagents and cells are combined to generate GEMs in such a way that single cells are partitioned with unique, cell-linked molecular barcodes. The simple workflow creates >90,000 GEMs per channel in an instrument run time of <18 minutes. Unique Platform Characteristics Generate single-cell transcript counts from up to 80,000 cells per run Produce >90,000 individual nanoliter-scale partitions leveraging ~3.6 million unique barcodes Capture cells at high efficiency up to 65% Low doublet rate of 0.9% per 1,000 cells. Achieve deep profiling of complex cell populations with high-throughput digital gene expression of individual cells Provide turn-key analysis pipelines and visualization software for novice and expert users alike Include a microfluidics chip that is compatible with a wide range of eukaryotic cell sizes and types (e.g. adherent cell cultures, suspension cell cultures, tissues, blood, etc.) Rapid time to lysis provides an accurate view of the transcriptome with minimal impact on expression Chromium Controller Specifications: Weight 12.5 lb (5.6 kg) Size (W X D X H) 7.9 x 10.3 x 6.4 in (20 x 26.3 x 16.4 cm) Electrical Requirements Nominal 100-240 Vac, 50-60 Hz, 15 W, from a standard 3- prong wall receptacle that includes a safety ground pin. Maximum 90-264V operational range (+/- 10% of Nominal). Overvoltage Category II (Standard Receptacle) Pollution Degree 2 (Indoor Use Only) Ventilation Requirement Minimum 4 in (10 cm) Around All Sides Usable Temperature Range 18-28 C (64-82 F) Humidity 85% Max (Non-Condensing) Altitude 0 6560 ft (0-2000 m) Packaging Information Weight 20 lbs (9.1 kg) Size (W X D X H) 16x16x12 in