Inactive Service-Disabled Veteran-Owned Small Business (SDVOSB) Set-Aside (FAR 19.14)

Request for Quote (RFQ) #: 36C25922Q0330 554 (C) Research_Plate Reader This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR Subpart 12.6, as supp... Request for Quote (RFQ) #: 36C25922Q0330 554 (C) Research_Plate Reader This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR Subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; quotes are being requested and a written solicitation will not be issued. The Government reserves the right to make no award from this solicitation. * The deadline for all questions is 16:00pm EST, May 10, 2022 All questions must be submitted in writing no later than the question cut-off date marked above. All questions will be answered officially through an amendment to the solicitation. ** Quotes are to be provided to Natasha Holland no later than 16:00pm ET, May 13, 2022 Quotes shall not be hand carried. Faxed quotes shall not be accepted. Emailed quotes are the only method of submitted quotes (4MB email limit). The Government reserves the right to make award solely on initial Quotes received. Offerors bear the burden of ensuring that all portions of the offer (and any authorized amendments) reach the designated office before the deadline specified in the solicitation. This RFQ shall be completed in its entirety, and signed and dated, failure shall not be considered for award. This is a Request for Quote (RFQ) and the solicitation number is (36C25922Q0330). The government anticipates awarding a firm-fixed price contract resulting from this solicitation. This solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Circular (FAC) 2021-06 effective July 12, 2021. The North American Industrial Classification System (NAICS) code for this procurement is (334510) with a small business size standard of (1250 employees). This solicitation is a 100% set-aside for (SDVOSB). List of Line Items; Line Item Description Quantity Unit of Measure Unit Price Total Price 0001 Agilent BioTek Synergy H1 Hybrid Multi-Mode Reader, Model: H1M2G, Incubator Temperature: 4-Zone incubation to 70 deg.C, Compatibility: BioStack and Third Party Automation Compatible, Detector Type: Photodiode, PMT, Light Source: Xenon Flash, CO2 Concentration Range: 0 to 20% Vendor Catalog # SH1M2G-SN Hazardous Material OR EQUIVALENT 1 EA $ $ 0002 Agilent BioTek Gas Controllers for Synergy Neo2 Multi-Mode Reader, Carbon-di-oxide and Oxygen Gas Controller, For Use With: For use with Lionheart, Neo2 and Cytation Vendor Catalog # 1210013 OR EQUIVALENT 1 EA 0003 Agilent BioTek Take3 and Take3 Trio Micro-Volume Microplates, No. of Positions: 16 Vendor Catalog # TAKE3 OR EQUIVALENT 1 EA Grand Total $ Description of Requirements for the items to be acquired in the Salient Characteristics below: The plate reader must support applications including Elisa, microbial growth assay, nucleic acid and protein quantification, TR-FRET, fluorescence polarization (FP), bioluminescence resonance energy transfer (BRET), enzyme kinetics, protein aggregation, cell-based assays, metabolic activity, reactive Oxygen Species (ROS). All the applications are required to meet the requirements of the research study. In addition, the plate reader must offer an extended dynamic range, which allows detection of signals across a 7 log measurement range. Temperature control to 70 °C, CO2 / O2 control, and a shaking platform create the required environmental controls for live cell assay workflows. General Detection Modes UV-Vis absorbance Fluorescence intensity Luminescence Fluorescence polarization Time-resolved fluorescence Read methods End point Kinetic Spectral scanning Well area scanning Microplate types 6- to 384-well plates Other labware supported Take3 Micro-Volume Plates Temperature control 4-zone incubation to 45C with condensation control +/-0.5C at 37C Shaking Linear Orbital Double orbital CO2 and O2 control Range 0-20% CO2 1-19% O2 Control resolution +0.1% (CO2 and O2) Stability +0.2% at 5% Cox +0.2% at 1% O2 Software Microplate Reader and Imager Software Secure for 21 CFR Part 11 compliance (option) Automation BioStack and 3rd party automation compatible BioSpa 8 Automated Incubator compatible Absorbance Light source: Xenon flash Detector: Photodiode Wavelength selection: Monochromator Wavelength range 230-999 nm 1nm increments Monochromator Bandwidth 4 nm: 230-285 nm 8 nm: >285 nm Wavelength Accuracy +/-2 nm Repeatability +/- 0.2 nm Dynamic range: 0 4.0 OD Path length correction: yes Optical density Accuracy <1% at 3.0 OD Reading speed (kinetic) 96 wells: 11 sec 384 wells: 22 sec Fluorescence Intensity Light source: Xenon flash Detector PMT for monochromator system PMT for filter system Wavelength selection Quad monochromators (top/bottom) Filters (top) Wavelength range Monochromators: 250 700 nm (900 nm option) Monochromator bandwidth Variable: from 9 to 50 nm, in 1 nm increments Fixed: 16 nm Dynamic range: 7 decades Sensitivity Filters Fluorescein 0.25 pM (0.025 fmol/well, 384-well plate) Quad Monochromator Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) top Fluorescein 4 pM (0.4 fmol/well, 384-well plate) bottom Reading speed (kinetic) 96 wells: 11 sec 384 wells: 22 sec Luminescence Sensitivity Monos: 20 amol ATP Filters: 10 amol ATP Fluorescence Polarization Light source: Xenon flash Detector: PMT Wavelength selection: Filters Wavelength range Excitation range: 330 700 nm Emission range: 400 700 nm Sensitivity: 2 mP standard deviation at 1 nM fluorescein Time-resolved Fluorescence Light source: Xenon flash Detector: PMT Wavelength selection: Filters (top) Sensitivity: <40 fM Reagent Injectors Supported detection modes: All modes Number: 2 syringe pumps Supported labware: 6- to 384-well plates Dead volume: 1.1 ml with back flush Dispense volume: 5 1000 uL in 1 uL increments Dispense accuracy: +/- 1 uL or 2% Dispense precision: