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The Department of Veterans Affairs Corporal Michael J. Crescenz VAMC, 3900 Woodland Ave, Philadelphia, PA 19104 is conducting a market survey to identify potential sources to provide a Tecan Microplat... The Department of Veterans Affairs Corporal Michael J. Crescenz VAMC, 3900 Woodland Ave, Philadelphia, PA 19104 is conducting a market survey to identify potential sources to provide a Tecan Microplate Reader per statement of need. DISCLAIMER This Sources sought is issued solely for information and planning purposes only and does not constitute a solicitation. Submitting information for this announcement is voluntary and VA assumes no responsibility for any costs incurred associated with the preparation of responses submitted as a result of this notice. If a solicitation is issued it will be announced at a later date, and all interested parties must respond to that solicitation announcement separately from the responses to this announcement. The purpose of this market research is to identify capable sources that are both experienced and qualified to provide Tecan Microplate Reader per statement of need. The North American Industry Classification System Code (NAICS) is 334516. Based on this information, please indicate whether your company would be a large or small business. Limitations on Subcontracting and non-manufacturing rule will apply to the resulting solicitation. Technical Data and Documentation: Companies are invited to provide their equipment specifications, warranty information, and manufacturer details. Sources must provide the following information: Company name, address, telephone number, website, DUNS number, CAGE code, business type, socio-economic status (e.g., SDVOSB, VOSB, Woman-Owned, Disadvantaged Small Business, 8(a), general small business, large, business, etc.), SAM registration expiration date, and person of contact (including telephone number and email address). Indicate country of origin of manufacturer. Indicate your company s size under NAICS 334516. Please indicate any government contracts held including government agency, contract number, dollar value, period of performance, and government contact information. In addition to the above submissions can include specifications, brochures, etc. as attachments. Information regarding your company s capabilities to provide such services are to be submitted electronically on or before Friday, 04/29/2022 by 3:00 PM (EST) addressed to McDaniel Brayboy III, email mcdaniel.brayboy@va.gov Statement of need: 1. Contracting Officer s Representative (COR). Name: Richard Moore Department: Research & Development Address: Corporal Michael J. Crescenz VAMC Attn: R&D bldg. 21 3900 Woodland Ave. Philadelphia, PA 19104 Phone Number: 215-823-5800 ext. 6020 Email address: Richard.moore7@va.gov 2. Contract Title. Purchase of an Tecan Microplate Reader to be used to support a VA Merit Funded Research Program in the BEERS Laboratory at the University of Pennsylvania and CMCVAMC 3. Background. This equipment will be used to support a VA Merit Review funded research program in the laboratory of Dr. Michael F. Beers, VA Staff Physician. The BEERS laboratory has a partial off site waiver to perform VA Research at the University of Pennsylvania (UPENN) and also works at CMCVAMC. Because many of the samples analyzed cannot be transported between VAMC and UPENN labs, this would be a dedicated piece of equipment that will be used frequently in the Beers lab to complete aims and objectives of the VA funded research Uses include the quantitative assessment of protein expression, cytokine elaboration, gene expression, and cell signaling in biological tissues, fluids, and cultured cell systems related to the study of various lung diseases affecting the Veteran population, including idiopathic pulmonary fibrosis, COVID19 acute lung injury, and COPD/emphysema. The multi-well plate readers is a powerful technology that permits simultaneous analysis of hundreds of samples for protein expression, cytokine elaboration, gene expression, and cell signaling through the use of colorimetric UV, or fluorescent dyes and the instrument captures the visible, UV, or fluorescent signal in up to 384 microwells each representing an individual experimental condition to allow multiple comparisons and high throughput screening of drug compounds or other interventions. Over the past several years, multiwall microplate readers with cellular imaging have become a leading tool for mechanistic discovery in biomedical research. 4. Scope. The contractor must be able to provide the following system and components: Main System: Multiwell plate reader with standard capability to analyze UV-VIS absorbance, cuvette port, fluorescence top reading (including intensity, TRF, FRET & HTRF), fluorescence bottom reading for adherent cells, glow luminescence, gas control module), automated microplate lid lifter, and large/small humidity chamber for evaporation control for live imaging There must be plate shaking and incubation from 4 deg C above ambient up to 42 deg C. The software package must measure end point, kinetics and multilabel assays with raw data export directly into Excel. Accessories and Components Needed for Full Functionality: 1) HSM (high speed monochromator) to allow full wavelength scanning of samples and custom excitation and absorption capabilities for all dyes. All fluorescence detection modes are tunable via monochromator with variable bandpass 5-50nm. 2) Full incubation capabilities with combined Temperature, CO2 (Carbon Dioxide), AND O2 (oxygen) blending. 3) Lid lifter Module used to temporarily remove the lid of the microplate to execute, e.g. injection steps or measurement steps within the workflow of a long term experiment thus avoiding sample evaporation. 4) Cell counting, viability, and confluence assessment using brightfield imaging of all wells. 5) Microinjector for addition of reagents inside the plate reader. 6) Software features needed include full qualitative and quantitative EIA analysis, all major curve fittings (e.g. point-to-point linear and non-linear regression, polynomial, logit-log, four and five-parameter fits), comprehensive QC function (such as plate-to-plate QC for trending of standards and controls displayed in a Levy-Jennings graph), a range of data import and export options (Excel, ASCII, ASTM), a range of kinetic data reduction features (such as mean and max slope, enzyme kinetics, etc.), 7) Expandability- Options/future upgrade choices must include fluorescence polarization, laser based Alpha technology, Te-Cool for sub-ambient temperature control, dual injectors for flash kinetic reactions, heating & stirring for the microinjectors. Start date: As soon as possible Length of Service: N/A Work Tasks and Deliverables Deliver and install all items listed above in Room 216, Stemmler Research Building, University of Pennsylvania, 3450 Hamilton Walk, Philadelphia, PA 19104.