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Transfection Equipment Statement of Need The Research Service at VAPHS is in need of equipment capable of performing transfection or artificially introducing nucleic acids into cells, utilizing means ... Transfection Equipment Statement of Need The Research Service at VAPHS is in need of equipment capable of performing transfection or artificially introducing nucleic acids into cells, utilizing means other than viral infection. Such introductions of foreign nucleic acid using various chemical, biological, or physical methods can result in a change of the properties of the cell, allowing the study of gene function and protein expression in the context of the cell. The Service seeks to purchase a Lonza Nucleofector 2b or equivalent. All quotes will be reviewed by the end user to determine the suitability for the intended use. Description The basic, single cuvette based NucleofectorTM System for efficient transfection of hard-to-transfect cell lines and primary cells with different substrates (e.g., DNA vectors or siRNA oligonucleotides) in low-throughput format Capabilities Highly efficient transfection of primary cells and cell lines Reliable results due to high viability and preservation of cell functionality Over 150 ready-to-use Optimized Protocols containing cell type-specific guidance Applications Low-throughput transfection in single cuvette format Transfection of plasmid DNA, siRNA, shRNA, miRNA, RNA and more, e.g., Morpholinos Transfection of peptides, proteins or small molecules Suited for bacteria transformation Technical Specifications Dimensions (w × d × h): 30 × 23 × 11 cm (11.81 × 9.06 × 4.33 in) Weight: 2.8 kg (6.2 lb) Power supply: 100 110 VAC or 230 VAC 50 60 Hz, self-regulating Power consumption: 50 VA/fuse T630mA L250V Protection: IP 20, EN 61010-1, UL 61010A-1