Inactive

SOURCES SOUGHT: This is a SOURCES SOUGHT NOTICE. The U.S. Geological Survey is conducting a MARKET SURVEY to determine the interest and capability of any firm eligible to compete under NAICS code 5413... SOURCES SOUGHT: This is a SOURCES SOUGHT NOTICE. The U.S. Geological Survey is conducting a MARKET SURVEY to determine the interest and capability of any firm eligible to compete under NAICS code 541380 with a Small Business Size Standard $16.5M for an upcoming requirement. The Molecular Ecology Laboratory would like to examine RNA-based gene prediction for two non- model bird species. THIS IS NOT A SOLICITATION. ALL COST ASSOCIATED WITH PROVIDING INFORMATION AS REQUESTED BY THIS SOURCES SOUGHT NOTICE SHALL BE THE RESPONSIBILITY OF THE CONTRACTOR. Submission is voluntary. For each species the contractor will perform RNA extractions from five tissue/blood samples and any associated quality control checks. The contractor will generate total RNA sequencing libraries and sequence the prepared libraries using high-throughput 'next-generation' sequencing-by-synthesis. The contractor will perform RNA extraction with a DNase digestion step and quality checks for sufficient concentration and purity on up to nine submitted tissue and blood samples. The contractor will prepare a standard RNA-seq library prep with mRNA enrichment through rRNA depletion for each sample type. Each sample type should be individually barcoded before multiplexing into one final library preparation. The contractor will sequence the multiplexed library via paired-end, 100-basepair sequencing-by synthesis, targeting a minimum of 100 million PE reads passing filter per sample. The contractor will return any unused tissue/blood and extracted RNA to the Molecular Ecology Laboratory. The government will provide up to five tissue/blood samples (50 -100 mg of liver, brain, spleen, small intestine, gonads, heart, lungs, and/or skeletal muscle collected in RNA/aterTissue Protect Tubes, and 50-75 ¿L fresh blood collected in RNA protect Animal Blood Tubes) per species for RNA extraction, standard RNA-Seq library prep and sequencing. The contractor will deliver genomic sequence data fastq files to the government, along with any associated sequencing quality and performance statistics. Data files will be made available for download for at least 30 days after sequencing is completed. The contractor will return any unused tissue/blood and extracted RNA to the Molecular Ecology Laboratory. A scope of work will be provided with any solicitation that may be issued. Responses to this announcement are requested from (1) SBA certified Small Business HubZone firms; (2) Small Business firms certified by a Small Business Administration (SBA) district or regional office for participation in the 8(a) program; (3) Service-Disabled Veteran-Owned small Business firms; (4) Small Businesses. In response to this announcement, please indicate your firm's socioeconomic status (e.g. small business, service-disabled veteran-owned, women owned, HUBZone, etc.) This information will assist the Government in determining how small businesses can fit into the acquisition strategy. A firm is considered small under NAICS code 541380 if their AVERAGE annual receipts for the preceding three (3) years were less than or equal to $16.5 million. Any information submitted is voluntary. If sufficient interest and capability is not received from firms in categories (1) through (4) above, any solicitation may be issued as unrestricted without further consideration. Interested small business firms shall submit (1) NAME, ADDRESS, and PHONE NUMBER OF THE FIRM (including DUNS NUMBER); (2) a CAPABILITY STATEMENT including (a) RECENT (within the last three fiscal years) RELEVANT (similar projects to this requirement) performance history (identify the name of the owner and their point(s) of contact with telephone and fax numbers, e-mail addresses; and (b) in what capacity (e.g., prime, subcontractor etc.) they performed the work; and (3) their SIZE STATUS. Your company must have a DUNs number and be registered on-line at the System for Award Management (SAM). You will also be required to provide your small business status information either via the RFP or the On-line Representations and Certifications (ORCA) also located at the SAM website before an award to your company can be made. Your on-line ORCA registration will cover any/all of your offers to the Federal Government, and only needs updated annually. All responses must be submitted NLT September 03, 2019 at 12:00pm MST via e-mail to: cjcarter@usgs.gov This is NOT a Request for Proposal (RFP) or Invitation for Bid (IFB) and does not constitute any commitment by the Government. Responses to this sources sought notice will be used by the Government to make appropriate acquisition decisions. All interested sources must respond to future solicitation announcements separately from responses to this market survey. Any solicitation that may be issued will be posted on the Federal Business Opportunities website and no paper copies will be provided.