Inactive Total Small Business Set-Aside (FAR 19.5)

This contract action is for the acquisition of supplies for which the Government intends to solicit and negotiate with only one source under the authority of Federal Acquisition Regulation (FAR) Subpa... This contract action is for the acquisition of supplies for which the Government intends to solicit and negotiate with only one source under the authority of Federal Acquisition Regulation (FAR) Subpart 13.106-1(b)(1). The National Institute of Environmental Health Science (NIEHS), Office of Acquisitions (OA) intends to award a single source purchase order to Applied StemCell, 521 Cottonwood Drive, Suite 111, Milpitas, CA 95035, for the acquisition of a Large Fragment Knock-In Using TARGATT Integrase. The Applied StemCell Large Fragment Knock-In Using TARGATT Integrase is required for generation of a transgenic mouse line encoding a Flp/Cre-dependent Vcre recombinase. The Development Neurobiology Group at the NIEHS will use this transgene in combination with viral constructs to study the functions of noradrenergic neurons in the mouse brain. The applicable North American industry Classification System (NAICS) Code for this procurement is 541714 “Research and Development in Biotechnology (except Nanobiotechnology).” Its associated Business Size Standard is $1,000.00. Line item 1. #AST-5021-1, TARGATT DNA Vector Design and Construction -Design and cloning of TARGATT plasmid. *Client supply DNA template* (Qty.1 each) (Timeline 4-8 weeks) Line item 2. #AST-5021-2, QC of Reagents for Microinjection -TARGATT plasmid QC by enzyme digest and/or sequencing -Plasmid scale-up and purification to give RNase-free plasmid (Qty 1 each) (Timeline 1-2 weeks) Line item 3. #AST-5021-3, Pronuclear Microinjection and Embryo Implantation (Qty. 1 each) (Timeline 1-2 weeks) Line item 4. #AST-5021-4, Animal Care, Housing and Genotyping -Housing and monitoring of F0 pups -Genotyping of pups by PCR and/or sequencing (Qty 1 each) (Timeline 2-3 months) Line item 5. #AST-5021-5, F1 Breeding and Genotyping -Breeding of putative F0 founders with wild-type mice -Care and housing of F1 offspring -Gnetyping of F1 pups by PCR and/or sequencing (Qty. 1 each) (Timeline 2-3 months) Line item 6. Shipping and Handling (Qty. 1 each) Delivery time: 12 months, ARO NIEHS will solicit and negotiate directly with Applied StemCell and no solicitation will be issued. This notice of intent is not a request for competitive proposals or quotations; however, all responsible sources may submit a bid, proposal, or quotation which shall be considered by the agency. Offerors must have an active registration in the System for Award Management (SAM) www.sam.gov. A determination by the Government not to compete this proposed requirement based on responses to this notice is solely within the discretion of the Government. Interested parties must express their interest and must be submitted in writing via email to Valarie K. Sims at sims3@niehs.nih.gov and are due by 10:00a.m., December 22, 2020. No faxed documents will be accepted. If no responsive statements are received by the due date, an award will be made to Applied StemCell.