Inactive

This is a Notice of Intent to award, not a request for Quotation. The National Institute of Allergy and Infectious Diseases (NIAID) of the National Institutes of Health (NIH) intends to negotiate on a... This is a Notice of Intent to award, not a request for Quotation. The National Institute of Allergy and Infectious Diseases (NIAID) of the National Institutes of Health (NIH) intends to negotiate on an other than full and open competition basis with Genewiz, LLC for the procurement of Immunogenomics 10x B-cell Single Cell Immuno-Profiling and related services. The Viral Pathogenesis Section (VPS) routinely surveys the dynamics of B-cell responses following Zika virus (or other heterologous flavivirus) infection or vaccination. We need efficient, accurate, sensitive and prompt ultra-deep sequencing and customized bioinformatics to provide comprehensive characterization of the immense diversity of B-cell receptors (BCR) repertoire, including the ability to pair heavy and light BCR chains at single-cell resolution. Genewiz 10X B-cell Single Cell Immuno-Profiling and related services includes single cell isolation via microfluidic technology with up to 65% cell capture efficiency and subsequent indexed sequencing on Illumina Sequencing 10X Configuration. Sequencing would include the processing of about 6,000 cells per sample, with 5,000 total number of reads per cell. They also offer a proprietary cell freezing protocol that maintains cell viability and the company also performs a dead cell removal process that improves data quality. Service includes Ph.D.-level consultation providing guidance before, during and after the project, as well as the automated mining of data to yield easy-to-interpret results that reduces analysis time for the researcher. Additionally, large data files are stored for up to six months and can easily be accessed and analyzed by 10x Genomics Cell Ranger and Loupe Cell Browser allowing for customization of the sequencing results and easy sequence transfer for downstream DNA gene synthesis, if needed. Additionally, the service can be paired with other Genewiz services such as Bulk Immuno-profiling and downstream DNA gene synthesis that streamlines data processing and analysis from a single study. Changing vendor choices at this stage within the research would cause variants with our current data results. For continuity of research, this is a sole source requirement. The Place of performance NIH NIAID, Bethesda, MD 20892 The statutory authority for this sole source requirement is 41 U.S.C.1901 (e) (2) as implemented by FAR 13.106?1 only one responsible source and no other supply of service will satisfy agency requirements. THIS IS NOT A REQUEST FOR QUOTES. All responsible sources that could provide comparable services may submit a capability statement that will be considered by email (subject line to reference NOI?RML?C?2088052 to Diana Rohlman at diana.rohlman@nih.gov by 8:00 AM Eastern Standard Time, September 11, 2021. All compatibility responses received by the closing date of this synopsis will be considered by the Government. A determination not to compete this requirement, based upon responses to this notice, is solely within the discretion of the Government.