Inactive

The National Institutes of Health, National Institute of Child Health and Development (NICHD) intends to award a purchase order without providing for full and open competition to Cyagen US INC., 2255 ... The National Institutes of Health, National Institute of Child Health and Development (NICHD) intends to award a purchase order without providing for full and open competition to Cyagen US INC., 2255 Martin Avenue, Suite E, Santa Clara, CA. 95050, USA, for generation of conditional knock-in/out mouse lines. Our research focuses on the development of the mammalian hematopoietic system. A long-term area of interest for our laboratory is the study of signal transduction molecules and pathways that regulate T lymphocyte maturation in the thymus. Currently, we are analyzing transgenic and conditional nonsignaling mutants of T cell antigen receptor (TCR) signaling subunits to evaluate the importance of individual TCR signaling chains and motifs at specific stages of T cell development and in mature T cells. We are also using gene profiling to identify proteins that are important for fine-tuning the T cell–signaling response in developing and mature T lymphocytes. In conjunction with checkpoint inhibitors for immunotherapy in humans, these molecules may also be targets. We are also investigating the function of new T cell–specific proteins that we identified by subtraction library screening. We have identified two gene candidates: LAX1 and SIT1, that we would like to make conditional knockin/knockout mouse models of to future investigate. LAX1 and SIT1 are involved in the T cell receptor pathways and are genes that are related to our ongoing Themis 1 & 2 and 6Y/6F projects in the lab. The Government needs services for generation of conditional knock-in/out mouse lines. Please see attached Pre-Solicitation NICHD-21-109.