Inactive

This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in Subpart 12.6 as supplemented with additional information included in this notice. This announcem... This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in Subpart 12.6 as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; proposals are being requested and a written solicitation will not be issued. The solicitation number is HHS-NIH-NIDA-CSS-75N95022R00045 and the solicitation is issued as a request for proposal (RFP). This acquisition is for a commercial item or service and is conducted under the authority of the Federal Acquisition Regulation (FAR) Part 13—Simplified Acquisition Procedures; and FAR Part 12—Acquisition of Commercial Items, and is not expected to exceed the simplified acquisition threshold. The solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Circular (FAC) Number 2022-04, with effective date 1/30/2022. The associated NAICS code is 325414 and the small business size standard is 1,250 employees. This requirement is full and open with no set-aside restrictions. This requirement is for the following: Statement of Need and Purpose: The purpose of this requirement is to design a CRISPRi sgRNA library that improves upon the current research protocols, resulting in more robust gene knockdown and allowing more compact screens, thus reducing the cost of future experimentations. Background Information and Objective: The objective of this requirement is to generate a pooled oligo library of sgRNAs for the purpose of performing CRISPRi survival screens in iPSC differentiated into neurons for the Neurodegenerative Initiative (iNDI) project. The iNDI project has engineered a set of iPSC lines. Using these engineered lines, the generation of this tool allows for a CRISPRi sgRNA library to improve upon the current research protocols. This will result in a more robust gene knockdown and allow more compact screens. This entails a multi-step cloning method performed at a large-scale to ensure that the final library contains all of the ~20,000 unique sgRNAs that are included within the oligo pools. This requires a high level of expertise and quality control. Project requirements: Requirement#1 Generate a cloned oligo pool library of iC20-dsgRNA-2xU6-UMI-bsd-BFP_1, 21554 customer-defined variants cloned into customer-supplied vector, 250 ug plasmid DNA to be delivered to the INDI researchers. The synthetic oligo production needs to be the same in form fit and function of existing oligos used in this research project to maintain continuity. Requirement#2 Generate a cloned oligo pool library, iC20-dsgRNA-2xU6-UMI-bsd-BFP_2, assembly of 13569 UMIs, and constant region into customer-supplied vector with custom quality control, to be delivered to the INDI researchers. The synthetic oligo production needs to be the same in form fit and function of existing oligos used in this research project to maintain continuity. The Government anticipates award of a firm fixed-price contract for this acquisition, and the anticipated period of performance is one month after award Delivery or Deliverables: For each of the of the supplied custom vector, vendor will produce 2 oligo pools creating one final library containing all of the guide RNAs and UMIs; ~20,000 unique guide RNAs and 13, 000 UMI’s that are being currently researched within the CARD initiative. Key Personnel: Vendor will provide a dedicated project manager to the government as a point of contact for the services. Section 508—Electronic and Information Technology Standards: The contractor shall comply with Section 508 of the Rehabilitation Act (29 U.S.C. § 794d), as amended by the Workforce Investment Act of 1998 (P.L. 105-220), August 7, 1998. The provision at FAR 52.252-1, Solicitation Provisions Incorporated by Reference (Feb 1998), applies to this acquisition. This solicitation incorporates one or more solicitation provisions by reference, with the same force and effect as if they were given in full text. Upon request, the Contracting Officer will make their full text available. The offeror is cautioned that the listed provisions may include blocks that must be completed by the offeror and submitted with its quotation or offer. In lieu of submitting the full text of those provisions, the offeror may identify the provision by paragraph identifier and provide the appropriate information with its quotation or offer. Also, the full text of a solicitation provision may be accessed electronically at these addresses: https://www.acquisition.gov/browse/index/far https://www.hhs.gov/grants/contracts/contract-policies-regulations/hhsar/index.html (End of provision) The following provisions apply to this acquisition and are incorporated by reference: FAR 52.204-7, System for Award Management (Oct 2018) FAR 52.204-16, Commercial and Government Entity Code Reporting (Aug 2020) FAR 52.212-1, Instructions to Offerors-Commercial Items (Nov 2021) FAR 52.212-3, Offeror Representations and Certifications-Commercial Items (Nov 2021) HHSAR 352.239-73, Electronic and Information Technology Accessibility Notice (December 18, 2015) The clause at FAR 52.252-2, Clauses Incorporated by Reference (Feb 1998), applies to this acquisition. This contract incorporates one or more clauses by reference, with the same force and effect as if they were given in full text. Upon request, the Contracting Officer will make their full text available. Also, the full text of a clause may be accessed electronically at these addresses: https://www.acquisition.gov/browse/index/far https://www.hhs.gov/grants/contracts/contract-policies-regulations/hhsar/index.html (End of clause) The following clauses apply to this acquisition and are incorporated by reference: FAR 52.204-13, System for Award Management Maintenance (Oct 2018) FAR 52.204-18, Commercial and Government Entity Code Maintenance (Aug 2020) FAR 52.212-4, Contract Terms and Conditions--Commercial Items (Nov 2021). Addendum to this FAR clause applies to this acquisition and is attached. HHSAR 352.222-70, Contractor Cooperation in Equal Employment Opportunity Investigations (December 18, 2015) HHSAR 352.239-74, Electronic and Information Technology Accessibility (December 18, 2015) The following provisions and clauses apply to this acquisition and are attached in full text. Offerors MUST complete the provisions at 52.204-24 and 52.204-26 and submit completed copies as separate documents with their proposal. FAR 52.204-24 Representation Regarding Certain Telecommunications and Video Surveillance Services or Equipment (Nov 2021) FAR 52.204-26 Covered Telecommunications Equipment or Services-Representation (Oct 2020) FAR 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders--Commercial Items (Jan 2022) NIH Invoice and Payment Provisions (Feb 2021) HHSAR 352.232-71, Electronic Submission of Payment Requests (Feb 2022) The provision at FAR clause 52.212-2, Evaluation – Commercial Items (Nov 2021), applies to this acquisition. The Government will award a contract resulting from this solicitation to the responsible offeror whose offer conforming to the solicitation will be most advantageous to the Government, price and other factors considered. The following factors shall be used to evaluate offers: (i) technical capability (ii) price; (iii) past performance. Technical and past performance, when combined, are significantly more important than cost or price. A written notice of award or acceptance of an offer, mailed or otherwise furnished to the successful offeror within the time for acceptance specified in the offer, shall result in a binding contract without further action by either party. Before the offer’s specified expiration time, the Government may accept an offer (or part of an offer), whether or not there are negotiations after its receipt, unless a written notice of withdrawal is received before award. The Offerors to include a completed copy of the provision at FAR clause 52.212-3, Offeror Representations and Certifications-Commercial Items (Nov 2021), with its offer. If the offeror has completed FAR clause 52.212-3 at www.sam.gov, then the offeror does not need to provide a completed copy with its offer. The clause at FAR 52.212-4, Contract Terms and Conditions-Commercial Items (Nov 2021), applies to this acquisition. Addendum to this FAR clause applies to this acquisition and is attached. The Defense Priorities and Allocations System (DPAS) are not applicable to this requirement. Responses to this solicitation must include clear and convincing evidence of the offeror’s capability of fulfilling the requirement as it relates to the technical capability The price proposal must include unit price, shipping and handling costs, delivery days after contract award, delivery terms, breakdown and rationale for proposed costs, and any other information or factors that may be considered in the award decision. The Unique Entity ID from SAM.gov, the Taxpayer Identification Number (TIN), and the certification of business size must be included in the response. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov. All offers must be received by 12:00 p.m., Eastern Standard Time, on April 28,2022 and reference Solicitation Number HHS-NIH-NIDA-CSS-75N95022R00045. Responses must be submitted electronically to Eric McKay, Contracting Officer, at eric.mckay@nih.gov. Fax responses will not be accepted.