Inactive Total Small Business Set-Aside (FAR 19.5)

Traditional next generation sequencing of whole genomes is done in short 75bp – 300bp fragments. However, these short reads are not optimal for the novel pathogens which FDA does not have much knowled... Traditional next generation sequencing of whole genomes is done in short 75bp – 300bp fragments. However, these short reads are not optimal for the novel pathogens which FDA does not have much knowledge about since the repetitive region is often longer than 300bp and short reads will not provide enough information to assemble the whole genomes. To completely assemble the whole genomes of novel pathogens with the associated antimicrobial resistance genes, an instrument with long read length sequencing technology is required. FDA CVM/OR/DAFM requires an instrument with long read length capability to perform our studies on the horizontally transferable antimicrobial resistance genes and their genetic background.