The U.S. Department of Health and Human Services requires a new, commercially available Zeiss Lattice SIM5 super-resolution light microscope for its National Cancer Institute's Center for Cancer Research Microscopy Core. The Core serves the imaging needs of NCI researchers to study the cell biology of cancer, and this specific microscope is needed to meet high demand for super-resolution imaging to visualize molecular interactions in live cells. The required system must include a high-speed scanner, dual sCMOS cameras, four specific lasers, a Piezo stage, incubation for live cells, and specific objective lenses. The performance location is the CCR Microscopy Core.