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Title: Acquisition of Flow Cytometry Instrument with autosampler Document Type: Combined Synopsis / Solicitation Notice Solicitation Number: 75N91021Q00263 Proposed Posted Date: 9/1/2021 Proposed Resp... Title: Acquisition of Flow Cytometry Instrument with autosampler Document Type: Combined Synopsis / Solicitation Notice Solicitation Number: 75N91021Q00263 Proposed Posted Date: 9/1/2021 Proposed Response Date: 9/7/2021 Classification Code: 66 – Instruments and laboratory equipment NAICS Code: 334516 – Analytical Laboratory Instrument Manufacturing Business Size Standard: 1000 Employees Contracting Office Address Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Office of Acquisitions, 9609 Medical Center Drive, Room 1E122, Bethesda, MD 20892, UNITED STATES. Description of Requirement The National Cancer Institute (NCI), Division of Cancer Epidemiology and Genetics (DCEG), Laboratory of Translational Genomics would like to procure a Flow Cytometry Instrument with autosampler. This is a combined synopsis/solicitation for commercial items, prepared in accordance with format in FAR 12.6 as supplemented with additional information included in this notice. This announcement constitutes the only solicitation and a separate written solicitation will not be issued. This solicitation: No. 75N91021Q00263 includes all applicable provisions and clauses in effect through FAR FAC 2021-06 (July 2021) simplified procedures for commercial items. The North American Industry Classification System code is 334516 and the business size standard is 1000 employees. Only one award will be made as a result of this solicitation. This will be awarded as a firm fixed price type contract. BACKGROUND The National Cancer Institute (NCI), Division of Cancer Epidemiology and Genetics (DCEG), Laboratory of Translational Genomics will use this equipment to develop new approaches to the study of the genetic basis of cancer and its outcomes. Specifically, DCEG is investigating the genetic basis of strong association signals identified in candidate gene association studies, loci identified by linkage analyses in high-risk families, or genome-wide association studies (GWAS). Flow Cytometry is a cell analysis technique used to measure the volume of cells in a rapidly flowing fluid stream as it passes in front of a viewing aperture. Flow cytometry offers researchers the ability to rapidly characterize and analyze millions of cells and can be used in a significant number of cell analysis applications, ranging from phenotyping to cell health and viability. The flow cytometer will be used for a variety of functional assays including the analysis of cell cycle progression, apoptosis, and purity of cell type fractionation. TYPE OF ORDER This will be issued as a Non-Severable Firm Fixed-Price Purchase Order. SPECIAL ORDER REQUIREMENTS PRODUCT FEATURES/SALIENT CHARACTERISTICS The following product features/characteristics are required for this Instrument: 3 laser flow cytometry instrument: 405nm (50mW), 488nm (50mW), 637nm (100mW) Must have flat-top lasers (not Gaussian), providing an intensity profile which is flat over most of the covered area, allowing a much wider window of alignment and leading to fewer service calls over the life of the instrument. Lasers much be in fixed alignment that does not require user adjustment Acoustic focusing technology that pre-focuses the samples within the sample stream prior to laser interrogation, allowing sample rates up to 1mL/minute. Must have at least 13 detection channels, including forward and side scatter. Allows option to add an additional laser at a later time. Sample analysis volume: 20 uL – 4 mL. Flow rate of 12.5 – 1,000 uL/minute, user adjustable. Provides electronic event rate of up to 35,000 events per second. Must utilize a positive displacement syringe pump for sample delivery providing accurate volumetric analysis and clog-resistance. Must have a Sample Recovery feature to recover unused samples. Active fluid sensing to alert users when additional fluids are needed All fluid reservoirs are stored within the instrument. Automated start-up, shutdown, and cleaning cycles Fluorescence sensitivity: ?80 molecules of equivalent soluble fluorochrome (MESF) for FITC, ?30 for PE, ?70 for APC photodiode detection of forward scatter with 488/10 nm bandpass filter Side scatter from PMT with 488/10 nm bandpass filter Computer workstation with Windows 10 and monitor. Software for operation, data collection: Displays real time acquisition status to see event rate and total number of events collected. User control of flow rate, gating and laser voltage. On-plot compensation tools. Fully customizable software layout to display a variety of plot types. Templates can be created that include instrument and compensation settings Autosampler to allow walk-away use of the instrument. Autosampler compatible with 96-, 384-, and deep-well plates Autosampler performs automated cleaning for shutdown. Autosampler mixes samples by aspiration One-click conversion between tubes and plates – no restart required DELIVERY / INSTALLATION The instrument shall be delivered to the Contracting Officer Representative (COR), within 30 days of purchase order award. 9615 Medical Center Drive Rockville MD 20850 A minimum 12-month manufacturer warranty must be included Shipping/Handling costs must be included in the quote. NCI will not be responsible in the event charges are not included in the quote. Installation charges must be included in the quote. Note: NCI plans to award a contract that has FOB origin delivery terms. TRAINING Installation and Training shall occur within 14 days of delivery. This purchase shall include two days of on-site training with a Field Applications Scientist, to include two full, non-consecutive days of maintenance and instrument and software training. Optimization of instrument settings, templates, workspace set up, etc., will also be included. WARRANTY The Contractor shall warrant that any instruments, equipment, components, or other supplies (“Supplies”) procured by this purchase are free of defects in design, material, or manufacture for a period of 12-months from date of installation. The Contractor shall warrant that all consumables or expendable parts are free of defects in design, material, or manufacture for a period of at least 90 calendar days from date of acceptance by the Government. Supplies which are repaired or replaced shall be warranted for the remainder of the initial warranty period or for 90-days – whichever is greater. 12-months of Technical Support shall be included in the warranty.