Inactive Total Small Business Set-Aside (FAR 19.5)

ERFORMANCE WORK STATEMENT The U.S. Naval Medical Research Unit No. 6 (NAMRU-6), an AFHSB-GEIS partner, leads multiple studies involving a myriad of sites located throughout Latin America. These studie... ERFORMANCE WORK STATEMENT The U.S. Naval Medical Research Unit No. 6 (NAMRU-6), an AFHSB-GEIS partner, leads multiple studies involving a myriad of sites located throughout Latin America. These studies involve testing for causes of acute febrile illness (AFI) and respiratory influenza-like illness (ILI) pathogens using the FilmArray platform. The mission of the Armed Forces Health Surveillance Division (AFHSD) - Global Emerging Infections Surveillance and Response System (GEIS) is to contribute to the protection of all Department of Defense healthcare beneficiaries (especially deployed US military personnel) and the global community through an integrated worldwide emerging infectious disease surveillance system. The Virology & Emerging Infections Department of the U.S. Naval Medical and Research Unit No. 6 (NAMRU-6) is seeking to purchase a next-generation sequencing platform, such as Illumina’s MiSeq DX, in order to identify and characterize pathogens of military relevance such as respiratory viruses and arboviruses. The above platform is intended for targeted sequencing of DNA libraries generated from nucleic acids extracted from samples such as peripheral whole blood. The instrument uses reagent kits designed for library preparation and sequencing. The library preparation kit should include a variety of steps that include enzymatic fragmentation, size selection, end-repair, a-tail and ligation step in the same reaction. The process should allow including dual index at the 5 and 3 prime end for later sample identification. Also, the library preparation kit should be designed to construct libraries with a low DNA input (~50ng) with master-mixed reagents ready to use. Similarly, the sequencing kit should allow running more than 150 sequencing cycles. Those cycles are needed to run paired-end libraries with a high quality performance. We expect to have more than 2.0Gb per sequencing run with high Q30 values. Finally, the sequencing kit should include an internal control to validate the sequencing run. All of the above sequencing process can only be performed on a MiSeq DX instrument.