Inactive

The Uniformed Services University of the Health Sciences (USU) is the Nation's federal health sciences university and is committed to excellence in military medicine and public health during peace and... The Uniformed Services University of the Health Sciences (USU) is the Nation's federal health sciences university and is committed to excellence in military medicine and public health during peace and war. We provide the Nation with health professionals dedicated to career service in the Department of Defense and the United States Public Health Service and with scientists who serve the common good. We serve the uniformed services and the Nation as an outstanding academic health sciences center with a worldwide perspective for education, research, service, and consultation; we are unique in relating these activities to military medicine, disaster medicine, and military medical readiness. USU is located in Bethesda, MD on the grounds of the Naval Support Activity Bethesda. Source: The University of California, Davis Justification: In accordance with FAR 13.106-1, The University of California, Davis is the only known source that can provided the requested research. USU intends to negotiate on a sole source basis with the University of California, Davis, located in Davis, California for research with mapping the virulence of Anaplasma phagocytophilum through transcriptional profiling in horses. This is not a request for quotes and a solicitation package will not be issued. In accordance with 5.207(c)(16) (ii) all responsible sources may submit a capability statement, proposal, or quotation, which shall be considered by the agency. Sources interested in responding to this requirement must be able to provide compelling evidence that they can provide the above mentioned requirement as specified. A determination by the government not to compete this proposed contractual action based upon responses received is solely within the discretion of the government. Responses must be received via email (jason.motala@usuhs.edu) by 3:00 p.m. ET on 3 May 2019. This research will be procured in accordance with the procedures of FAR Part 12-Commercial Items and FAR Part 13-Simplified Acquisition Procedures.

Inactive

THIS IS NOT A REQUEST FOR QUOTES - THIS IS A REQUEST FOR INFORMATION AND INFORMATION SUBMITTED WILL BE USED FOR MARKET RESEARCH PURPOSES ONLY The Uniformed Services University of the Health Sciences (... THIS IS NOT A REQUEST FOR QUOTES - THIS IS A REQUEST FOR INFORMATION AND INFORMATION SUBMITTED WILL BE USED FOR MARKET RESEARCH PURPOSES ONLY The Uniformed Services University of the Health Sciences (USU) is the Nation's federal health sciences university and is committed to excellence in military medicine and public health during peace and war. We provide the Nation with health professionals dedicated to career service in the Department of Defense and the United States Public Health Service and with scientists who serve the common good. We serve the uniformed services and the Nation as an outstanding academic health sciences center with a worldwide perspective for education, research, service, and consultation; we are unique in relating these activities to military medicine, disaster medicine, and military medical readiness. USU is located in Bethesda, MD on the grounds of the Naval Support Activity Bethesda. Additional information regarding USU can be found on our website: www.usuhs.edu. USU has a requirement for a Mapping the virulence of Anaplasma phagocytophilum through transcriptional profiling in horses. Required Specifications: Interested offerors responding to this RFI, must provide information on whether they could quote the requested research listed below. Research Requirements: Screen and select horses 1. Choose 14 candidate horses. 2. Collect blood into EDTA from any proposed candidates. 3. Identify horses that are seronegative by IFA and PCR-negative. 4. Pre-examine first 7 horses: baseline heart rate; rectal temperature; respiratory rate.; body condition; other complete physical examination; collect blood into EDTA for baseline CBC, chemistry panel, and RNAseq. Phase I: Infect first 7 horses 5. Pre-medicate horses 10 minutes before inoculum delivery (5 horses with infectious 106 HL-60 cells with high-passage A. phagocytophilum Webster strain and 2 horses with uninfected HL-60 cells) slowly by IV. Monitoring and sample collection 6. Evaluate the horses post-infection. a. Check the horses every day for fever (> 102F). b. Collect blood for CBC, chemistry on days 4, 7, 9, 12, 14, 17, and 21. On day 12 submit ANA and serology. c. On day 5, start checking the horses 2x daily for fever. d. Take clinical assessments. e. Take blood daily in EDTA, submit for buffy coat analysis and A. phagocytophilum quantitative PCR. f. As soon as horse has fever > 105F or >5% infected neutrophils on buffy coat or CT <30, collect blood for second inoculation round. 7. Treat the horse with 10 ml oxytetracycline in 250 ml D5W. Phase II inoculation 8. Keep Phase I horse blood at room temperature. Based on CBC/buffy coat, calculate volume that contains 106 infected neutrophils. For controls, calculate volume of blood that contains 106 neutrophils. 9. Prepare second set of 7 horses as described above; inoculate 5 horses with warm inoculum from Phase I, monitor as for Phase I. Inoculate 2 control horses with warm blood from Phase I control horses. 10. Alert CEH that horses may become more clinically ill. 11. Collect blood as described for Phase I except collect 1L per horse. 12. Treat horses with oxytetracycline. Prepare samples for shipping 13. Process blood for shipping to USU. If you are interested in this requirement, please answer the following questions: 1. Are the specifications of research listed clear? 2. Are you able to provide the requested research identified in the in the above specifications? If no, what specifications could you not meet? 3. Is this research done by yourself or another company? 4. If you are a large business, do you have any authorized small business resellers that you work with? Please provide the following with your submission: 1. Company name and address 2. DUNS Number 3. Socio-Economic Status (Small, SDVOSB, etc.) - Socio-economic status will be verified in SAM.gov. 4. Point of Contact (Name, Email and Phone Number) All responses are due by 12:00 pm (Noon) ET on 15 April 2019 and must be emailed to jason.motala@usuhs.edu. You will receive confirmation that your submission was received by COB insert date. If you do not receive confirmation, please follow up with the contract specialist.