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The National Institute of Standards and Technology (NIST) intends to negotiate on a sole source basis, under the authority of FAR 13.5, Combinati Incorporated, 2450 Embarcadero Way Palo Alto, CA 94303... The National Institute of Standards and Technology (NIST) intends to negotiate on a sole source basis, under the authority of FAR 13.5, Combinati Incorporated, 2450 Embarcadero Way Palo Alto, CA 94303 for a Combinati Absolute Q Digital PCR for the National Institute for standards and Technology (NIST) from: SI-traceability is crucial to the Standard Reference Material (SRM) development work performed by the Applied Genetics Group(AGG). To make SI-traceable measurements, AGG must be able to assess and measure the partition volume, which can be done with the Combinati Absolute Q instrument, using Scanning Electron Microscopy (SEM) from discussions with scientists at other national metrology institutes and other market research, only the physical partitioning method employed by Combinati may be measured reliably in this way. The Applied Genetics Group has performed pre-market (beta test) research on the Combinati instrument using our SARS-CoV-2 test material and found that it can detect multiple targets using different dye channels simultaneously, with concordant results to the Bio-Rad QX200. Orthogonal measurements increase reliability and robustness of certified values for NIST SRM. Given its potential for SI-traceability, orthogonal methodology, and its multi-dye compatibility, only the Combinati Absolute Q meets the government’s requirements. Market research has shown that Combinati is the sole company who can provide this equipment to NIST. Given these facts, Combinati is the only source reasonably available to provide the required equipment. The resulting purchase order will be a Firm Fixed Price purchase order. The NAICS Code is 334516 with a size standard of 1,000 employees. No solicitation package will be issued. This notice of intent is not a request for competitive quotations; however, interested parties who regard themselves as capable of fulfilling these requirements are invited to submit responses to the Government for consideration. Responses received by 11:00 am EST Aug 31th 2021, will be considered by the Government. A determination by the Government not to compete the proposed acquisition based upon responses to this notice is solely within the discretion of the Government. Information received will normally be considered solely for the purpose of determining whether to conduct a competitive procurement. Inquiry POCs: Donald.Graham@nist.gov National Institutes of Standards and Technologies NIST