Inactive Total Small Business Set-Aside (FAR 19.5)

The USDA intends to award a sole source firm fixed price purchase order to EPOCH Life Science Inc. The USDA, ARS, Plum Island Animal Disease Center (PIADC) in Orient Point, NY, requires custom gene sy... The USDA intends to award a sole source firm fixed price purchase order to EPOCH Life Science Inc. The USDA, ARS, Plum Island Animal Disease Center (PIADC) in Orient Point, NY, requires custom gene synthesis and mutations to already synthesized genes to meet our research requirements for Classical Swine Fever Virus (CSFV) and African swine fever virus (ASFV). The vendor is provided a text file with what we want as our end result, the vendor then compares the end result with previous gene synthesis products to determine the most cost effective one to use as a starting point. The vendor then using a combination of new gene synthesis and re-using old gene synthesis builds our construct, in the desired expression vector which we specify. (See Attached for additional information)

Inactive Total Small Business Set-Aside (FAR 19.5)

The FADRU has a requirement for gene synthesis to conduct molecular biology research and plasmid biorepository at Plum Island Animal Disease Center. Included in this requirement is custom gene synthes... The FADRU has a requirement for gene synthesis to conduct molecular biology research and plasmid biorepository at Plum Island Animal Disease Center. Included in this requirement is custom gene synthesis, and gene point mutations. The proposed contract will support all gene synthesis needs. Individual requirements for gene synthesis will be submitted to the contractor. Due to the infinite potential gene synthesis involving any combination of nucleotides, individual project pricing will be based upon the agreed-upon pricing schedule along with project by project negotiation. Final project pricing will depend on the complexity of the requested gene variant. Complexity is defined and determined based on the following three factors: Secondary structure of requested variant, non-double helix DNA structures are harder to synthesize; Guanine-cytosine (GC) content of the DNA: a higher percentage of GC relative to adenine and thymine (AT) increases complexity and price; Length of new synthesis in the variant 4) Length separating requested changes when more than one area are variants. The above three factors consider the complexity of the gene variant, and are not independent variables, but variables that when taken together in the context of a variant, increase the complexity of the gene synthesis. Other factors that can decrease price are when variants are a series that are dependent on each other, in simple terms variant 1 has mutation 1, variant 2 has mutation1+2, variant 3 has mutation 1, 2, & 3. In this situation mutant 3 has three mutations, but only one mutation when using mutant 2 as a reference. In gene variant or mutagenesis pricing, this would constitute a group of variants, and would be discounted over a sole variant. In this case pricing will still depend on the complexity of the gene variant has defined but taking into consideration the group.