Inactive

This is a Sources Sought notice Only. This is NOT a solicitation for proposals, proposal abstracts, or quotations. This Sources Sought is issued for information and planning purposes only as part of U... This is a Sources Sought notice Only. This is NOT a solicitation for proposals, proposal abstracts, or quotations. This Sources Sought is issued for information and planning purposes only as part of USDA ARS market research and does not itself constitute an RFQ. This requirement is covered under NAICS code 32513. The purpose of this notice is to obtain information regarding the availability and capability of qualified sources; Small Businesses; HUBZone small business; service-disabled, veteran-owned small business; 8(a) small business; veteran-owned small business; woman-owned small business; or small disadvantaged businesses; Responses to the information requested will assist the Government in determining the appropriate acquisition method, including whether a set-aside is possible. The project investigates the genetic regulation of a specific metabolite and atherosclerosis. Whole genome genotyping using a commercial microarray is needed. To ensure adequate data depth a minimum of 143,000 single nucleotide polymorphisms (SNPs) need to be assayed. These SNPs should maximize genetic diversity by including SNPs from over 150 inbred mouse lines. the SNPs should also be space at 22.5kb on average across the genome to ensure adequate coverage. Services should include DNA extraction. To ensure adequate data depth a minimum of 143,000 single nucleotide polymorphisms (SNPs) need to be assayed. These SNPs should maximize genetic diversity by including SNPs from over 150 inbred mouse lines. the SNPs should also be space at 22.5kb on average across the genome to ensure adequate coverage. Services should include DNA extraction. Output of these assays should be genotyping data in a format acceptable for use in the qtl2 analysis program. The awardee will utilize inhouse personnel to perform all aspects of sample preparation and running the genotyping assays per manufacturer’s specifications. We will provide mouse tail samples for the proposed assays. These will be shipped on dry ice with the manifest information needed to run the assays and a sample sheet We anticipate shipping the samples for genotyping within 4 weeks of award. Upon receipt Neogene will process the samples. Specifically they will isolate DNA and perform genotyping within 4 weeks of receipt. They will supply data within 6 weeks the following items necessary for genotype analysis. Note- samples will be exhausted in this process and will not be returned. sample map- a text file with the following headers: Index Name ID Gender Plate Well Group Parent1 Parent2 Replicate SentrixPosition snp map- a text file with the following headers: Index Name Chromosome Position GenTrain Score SNP ILMN Strand Customer Strand NormID DNA report- a text file with the following headers: Row,DNA_ID,#No_Calls,#Calls,Call_Rate,A/A_Freq,A/B_Freq,B/B_Freq,Minor_Freq,50%_GC_Score,10%_GC_Score,0/1 Locus summary a text file with the following headers: Row, Locus_Name, Illumicode_Name , #No_Calls, #Calls, Call_Freq, A/A_Freq, A/B_Freq,B/B_Freq, Minor_Freq, Gentrain_Score, 50%_GC_Score, 10%_GC_Score, Het_Excess_Freq, ChiTest_P100, Cluster_Sep, AA_T_Mean, AA_T_Std, AB_T_Mean, AB_T_Std, BB_T_Mean, BB_T_Std, AA_R_Mean, AA_R_Std, AB_R_Mean, AB_R_Std, BB_R_Mean, BB_R_Std, Plus/Minus Strand Locus by DNA report Final report a text file with the following headers: SNP Name Sample ID Allele1 - Forward Allele2 - Forward Allele1 - Top Allele2 - Top Allele1 - AB Allele2 - AB GC Score X Y Data Rights: The data will be the sole property of the USDA upon completion of the analysis. The Government does not intend to make an award based on responses to this inquiry. This notice does not constitute a commitment by the Government. Capability Statement All information submitted in response to this announcement is voluntary, and the Government will not pay for information requested nor will it compensate any respondent for any cost incurred in developing information provided to the Government. Business concerns that possess the capabilities necessary to undertake this work should submit complete documentation of their capabilities to the Contracting Officer. Capability statements shall include the Contractors contact point to discuss their response. The capability statement should include: 1) Acknowledgement that they are able to provide the subject supplies/services being requested; 2) Interested vendors should identify their size standards in accordance with the Small Business Administration; 3) At least three previous requirements that are similar to the requirement include a discussion of the Contract type, delivery method, duration, etc.; 4) Any other information considered relevant to this requirement; and 5) The government requests that no proprietary, classified, confidential, or sensitive information should be included in the response. Any vendor responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine their qualifications to perform the work. Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After review of the responses received, a combined synopsis/solicitation may be published on sam.gov depending on the responses received. All responses to this Sources Sought Notice must be received by the notice’s POC by the deadline in order to be considered. Responses may not be returned. Responders are solely responsible for all expenses associated with responding to this Inquiry. Do not submit pricing information in response to this Request.