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Electroantennogram (GC coupled EAG) assembly Electroantennography (EAG) is a bioassay widely used in experimental entomology for the detection of volatiles perceived by the antennal olfactory apparatu... Electroantennogram (GC coupled EAG) assembly Electroantennography (EAG) is a bioassay widely used in experimental entomology for the detection of volatiles perceived by the antennal olfactory apparatus of insects. The EAG signal is a voltage deflection, which can be measured with suitable equipment, between the tip and the base of an insect antenna when exposed to an adequate stimulus. Recording the delicate voltage fluctuations across the antenna requires a sensitive instrument, which does not interfere with the physiological processes in the antenna. The small voltage fluctuations need to by carefully picked-up and amplified to a sufficiently high level to drive a recording device, such as an oscilloscope, chart recorder, or computer system.