Inactive

The United States Department of Agriculture (USDA), ARS, Forage and Range Research Utah in Logan, UT has a requirement for genome sequencing and TAD analysis, including DeNovo Assembly QC, a Proximity... The United States Department of Agriculture (USDA), ARS, Forage and Range Research Utah in Logan, UT has a requirement for genome sequencing and TAD analysis, including DeNovo Assembly QC, a Proximity Ligation Library Prep and sequencing for 133Gb, and an assembly Informatics package. The samples to be analyzed are bluebunch wheatgrass. Delivery is required within 16 weeks from award. The NAICS Code is 541715 . The PSC will be B529. This is a combined solicitation and synopsis for the U. S. Department of Agriculture (USDA), Agricultural Research Service (ARS), Forage and Range Research Utah in Logan, Utah. This is a request for quotations for the genomic scaffolding for two perennial grass genomes. This announcement is open all interested and capable offerors. The USDA is seeking a Firm Fixed Price proposal from a qualified Contractor for the genome sequencing and TAD analysis, including DeNovo Assembly QC, a Proximity Ligation Library Prep and sequencing for 133Gb, and an assembly Informatics package. The samples to be analyzed are bluebunch wheatgrass. The following are the input constraints for TAD analysis: 40% of read pairs should map at a distance > 1Kbp Genome size < 10Gb Assembly scaffold size < 400Mbp >40% of genome uniquly mappable Coverage: 100 M read pairs per 1Gb Deliverables shall include: • HiRise Assembly deliverables include: • The HiRise assembly in FASTA format • A report summarizing key assembly statistics, features of the proximity ligation library, and a linkage density plot of the proximity ligation library data • A table detailing the breaks made to the input scaffolds • A table describing the position of the input assembly scaffolds within the final HiRise scaffolds • BAM file(s) containing alignments of the proximity ligation library read pairs mapped to the draft assembly • TAD Analysis deliverables, if applicable, include: • TAD calls at 10kbp, 25kbp, and 50kbp • CTCF binding sites • A/B compartmental organization • Isochore locations • Guide to loading and viewing data in HiGlass • Summary report As authorized by FAR 5.102, solicitation dissemination is limited to electronic medium, and the USDA will provide no hard copies. The solicitation will only be available for download at no charge at the Contract Opportunities website (https://beta.sam.gov/). Offeror's are advised that they are responsible for obtaining ALL solicitation documents and acknowledging any amendments and should re-visit the Contract Opportunities (https://beta.sam.gov/) website periodically to check for any modifications to this notice, updates, or amendments without further notice from the USDA. Prospective offerors shall complete electronic annual representations and certifications at http://www.sam.gov/ (SAM), shall be registered in the System for Award Management (SAM) database at http://www.sam.gov prior to submitting an offer. Address all questions in writing to the issuing office via email to: shelley.steen@usda.gov no later than March 11, 2021 at 3pm PST. No telephone inquiries will be accepted.

Inactive

THIS IS A SOURCES SOUGHT ANNOUNCEMENT ONLY. This notice does not constitute a commitment by the Government. All information submitted in response to this announcement is voluntary, and the Government ... THIS IS A SOURCES SOUGHT ANNOUNCEMENT ONLY. This notice does not constitute a commitment by the Government. All information submitted in response to this announcement is voluntary, and the Government will not pay for information requested nor will it compensate any respondent for any cost incurred in developing information provided to the Government. The United States Department of Agriculture (USDA), ARS, Forage and Range Research Utah in Logan, UT (FRRL) has a requirement genomic scaffolding of two perennial grass genomes, bluebunch wheatgrass at 5Gb size and orchardgrass at 2Gb size. FRRL can ship fresh tissue as well as a contig level assembly. They need high molecular weight DNA extraction, proximity ligation with an endonuclease to ensure even coverage, and scaffolding assembly. The purpose of this sources sought notice is to determine if there are adequate vendors available capable of providing the requirement. The NAICS Code is 541715 The PSC will be B529 In response to this sources sought notice please submit information on your company's capabilities to provide this type of service Please also provide information on whether your company is the manufacturer or a distributor and a small or large business in accordance with the NAICS code listed in this sources sought notice. The Government acknowledges that responses to this sources sought notice are not binding offers. The information obtained will assist in our market research and future solicitation processes. The purpose of this sources sought is solely to obtain information and sources for our future requirements. No Request For Quote (RFQ) exists. Responses to the Sources Sought will not be returned. Do not submit pricing information in response to this Sources Sought. Please send responses with the subject heading 1026976, Bluebunch Wheatgrass Genomes to Shelley Steen at shelley.steen@usda.gov by Friday, March 5, 2021 at 5pm EST.