PacBio SMRT sequencing kits (Brand Name restriction)
Acquisition Description: This notice is a combined synopsis/solicitation for commercial items using Simplified Acquisition Procedures. This acquisition will be made in accordance with the format in Fe...
Acquisition Description: This notice is a combined synopsis/solicitation for commercial items using Simplified Acquisition Procedures. This acquisition will be made in accordance with the format in Federal Acquisition Regulation (FAR) Subpart 12.6 “Streamlined Procedures for Evaluation and solicitation for commercial Items,” as applicable, and as supplemented with additional information included in this notice. This announcement constitutes the only solicitation (a written solicitation will not be issued) and quotes are being requested. This acquisition will be processed under FAR Part 13 Simplified Acquisition Procedures (SAP). The solicitation documents and incorporated provisions and clauses are those in effect through Federal Acquisition Circular (FAC) 2022-07, August 10, 2022. The North American Industry Classification System (NAICS) code for this procurement is 334516 - Analytical Laboratory Instrument Manufacturing, with a small business size standard of 1000 employees. The combined synopsis/solicitation number is RFQ-NIAID-2134335 and this is issued as a Request for Quotes (RFQ), under a Brand Name restriction (considered limited source restriction). This requirement IS a TOTAL SET-ASIDE for small business. The vendors who submit a response to this RFQ must be an authorized seller/reseller of these products. BRAND NAME: NIAID requests responses from qualified sources capable of providing the requirements as outlined. To be considered for award, vendors must provide the exact brand and model number of the product from the required brand-name owner/manufacturer, along with specific services and/or warranties that typically comes with these items when purchased directly from the owner/manufacturer. Project Description: National Institute of Allergy & Infectious Diseases (NIAID)’s Vaccine Research Center Virus Persistence and Dynamics Section (VPDS) (collectively “Program”) works to analyze the mechanisms of virus persistence in vivo, with the aim of informing development of vaccines, immune-based therapies, and other medical countermeasures for virus-mediated diseases. A central mechanism of virus persistence is mutational variation, which can lead to antigenic variation and present an obstacle to naturally-acquired, vaccine-elicited, or passively-administered immunity. Program has developed technology termed high-throughput, single-genome amplification and sequencing (HT-SGS), which uses long-read deep sequencing to describe genetic variation among virus genomes within individual samples. Applied to longitudinal samples across multiple people, experimental animals, or other infection models, HT-SGS can reveal important patterns of virus mutational variation and evolution. In order to accomplish its research goals with HT-SGS, Program requires the SMRT sequencing kits and reagents from Pacific Biosciences of California Inc. (“PacBio” or “Pacific Biosciences”) . Requirements: Brand Name Restriction items: 1. 100-265-900 AMPure PB beads - Specially formulated AMPure PB beads supplied at 5 mL volume. Qty: 52 2. 102-009-200 SMRTbell barcoded adapter plate 3.0 - 96 SMRTbell barcoded overhang adapters in plate format (1 sample per barcoded adapter). Includes barcodes bc2001–bc2096. Qty: 28 3. 101-842-900 Sequel II binding kit 2.0 - Reagents for binding sequencing polymerase to SMRTbell library (24 samples). Includes Sequel II internal control complex 1.0. To be used for samples with inserts >/= 3 kb. Qty: 10 4. 101-843-000 Sequel II binding kit 2.1 - Reagents for binding sequencing polymerase to SMRTbell library (24 samples). Includes Sequel II internal control complex 1.0. To be used for samples with inserts <3 kb such as smaller amplicons. Qty: 4 5. 101-820-200 Sequel II sequencing kit 2.0 - Sequencing reagent plate supporting 4 SMRT Cells 8M. Qty: 42 6. 101-389-001 SMRT Cell 8M tray - Sequel II and IIe system SMRT Cell tray (4 SMRT Cells 8M). Qty: 42 7. 100-621-300 SMRT Cell oil - 5 tubes of SMRT Cell oil for the Sequel and Sequel II systems, each supporting up to 4 SMRT Cells (20 SMRT Cells). Qty: 9 Submission must include Shipping and Handling. Place of Performance or Delivery: NIAID-Vaccine Research Center 40 Convent Drive Attn: Liliana Perez Rodriguez Bethesda, MD 20814 FOB: Destination The government intends to award a firm fixed price purchase order to the responsible contractor as a result of this RFQ that will include the terms and conditions set forth herein. By submitting an offer in response to this RFQ, confirms acceptance that the government terms and conditions will prevail over the award. The award will be based on the following: the capability to meet the brand name requirement and price. Submission shall be received not later than August 28, 2022 @ 4:00 PM EST Offers may be e-mailed to Lu Chang at
Data sourced from SAM.gov.
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