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The Laboratory of Cellular and Developmental Biology of the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) has a requirement to purchase a BD Accuri C6 Plus flow cytometry sy... The Laboratory of Cellular and Developmental Biology of the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) has a requirement to purchase a BD Accuri C6 Plus flow cytometry system. The contractor shall provide the following to brand name specifications: The features of the BD Accuri C6 Plus that meet our critical needs are as follows: 7.2-decade dynamic range (16 million channels of digital data) Highly sensitive: MESF <75 (FITC) and <50 (PE)1 Non-pressurized peristaltic pump system Event rates up to 10,000 events per second Small footprint Uses laboratory-grade water as sheath fluid Blue and red laser, two light scatter detectors, and four fluorescence detectors with optical filters optimized for the detection of fluorochromes, including FITC, PE, PerCP-Cy™5.5, and APC, as well as newer polymer dyes Analyzes variants of fluorescent proteins, such as GFP, YFP, and mCherry. Software allows visualization of data at any scale, so that users can precisely set gates and regions. Can calculate absolute counts or sample concentration per ?L without the use of counting beads Automated sampler that supports 48-well plates 96-well plates 96-deep-well plates 24-tube rack (included) for standard 12 x 75-mm tubes Software features free downloadable software templates for many assays and applications Automated instrument quality control Analyzes a variety of cellular processes: Apoptosis Cell signaling DNA content DNA damage Cell cycle Proliferation Viability Facilitates multiple aspects of stem cell research from the assessment of pluripotent and adult stem cell phenotypes to iPSC reprogramming efficiency.